In Then, representative DACSes based on DEP trapping, traveling
In 2017 Angela Babetski Holton et all
design a specific microfloudics trapping
devices for real time monitoring of cancer cell1.
2017 Mario Rothbauer et. al have studied
recent advanced technology for making 2D or 3D of cancer cell their
interaction and they also developed
advanced micro devices to intrigrate multiple organs for human body2.
In 2017 Uday K. Veeramallu explore that the MetaCellTM separators have the potential to serve as
very effective adjunctive aids for the selection and monitoring of ovarian
cancer patients for treatment with targeted therapies3.
In 2017 Paridhi Puri,
Vijay Kumar, M. Ananthasubramanian et al Design, simulation and fabrication of MEMS based
dielectrophoretic separator for bio-particles. Also found the
simulation results for the optimization of dielectrophoretic device is
presented and discussed in detail. Simulation results for cell separation is
carried out in a circular channel driven by AC field and the process model with
fabricated device. Compared with the previously reported dielectrophoretic
separation devices, this structure achieves continuous separation and also
minimizes dead volumes4.
In 2017, Muhamad R. Buyong; Farhad Larki; Yuzuru
Takamura; Burhanuddin Yeop Majlispresents explore the fabrication, characterization,
and simulation of microelectrode arrays system with tapered profile having an
aluminum surface for dielectrophoresis (DEP)-based manipulation of particles.
The proposed structure demonstrates more effective electric field gradient
compared with its counterpart with untapered profile. Therefore, according to
the asymmetric distribution of the electric field in the active region of
microelectrode, it produces more effective particle manipulation5.
Bahareh Haddadi, Morteza Fathipour
perform the numerical analysis of 3D
micro floudic device model of cell separator having different densities by
using Standing Surface Acoustic Waves6 .
In 2016, Aissa Foughalia, S. Noorjannah Ibrahim explore separation efficiency of the microfluidic device in continuous flow
based on results of a 3D model simulation conducted in COMSOL Multiphysics. The
proposed device enables label-free cell separation and thus, it can be a useful
microfluidic component for lab-on-a-chip system and integrated biological and
biomedical applications 7.
Lee et al explores current DACS capabilities worldwide, and it also looks at
recent developments intended to overcome particular limitations. First, the
basic theories are reviewed. Then, representative DACSes based on DEP trapping,
traveling wave DEP systems, DEP field-flow fractionation and DEP barriers are
introduced, and the strong and weak points of each DACS are discussed. Finally,
for the purposes of commercialization, prerequisites regarding throughput,
efficiency and recovery rates are discussed in detail through comparisons with
commercial cell sorters (e.g. fluorescent activated and magnetic activated cell
In 2016, A Heidari
perform the Comparative Study on Simultaneous Determination and Separation of
Adsorbed Cadmium Oxide (CdO) Nanoparticles on DNA/RNA of Human Cancer Cells
Using Biospectroscopic Techniques and Dielectrophoresis (DEP) Method 9.
In 2016, Mehdi Sahmani , Mousa
Vatanmakanian , Mehdi et al established that the several characteristics of a microchip can
under shadow its overall functions which are include: the physics of the chip
and its dimensions; material used in its synthesis; types of pumps, channels
and valves; chip manufacturing technology; the type, quantity, quality and
sample processing, reliability, standardization, precision and the sensitivity
of chip for sampling, analysis and final recording of the results. In the near
future each of these features might be a topic for different challenges and investigations.
Conclusively, further development of ingeniously designed micro-systems,
identification of proper materials with a high bio-compatibility for chip
manufacturing, discovering novel biomarkers, optimizing the integration of
various processes on a single platform, and using highly efficient
bio-detectors in the structure of microchips can help to largely enhance the
early diagnosis of human cancers. In fact, in the current scientific community
which with the advent of modern technology and advanced equipments is rapidly
developing, chip-based cancer monitoring like the other large research projects
has been turned out to an extensive field of study and has been able to obviate
many concerns regarding the human cancers and the related challenges. It is
valuable to note that this trend is progressively going through forward which
can bring promising achievements for health-care system10.
In 2015, Peng Lia , Zhangming Maoa ,
Zhangli Peng et al validated the capability of this device by successfully
separating low concentrations (?100
cells/mL) of a variety of cancer cells from cell culture lines from WBCs with a
recovery rate better than 83%. We then demonstrated the isolation of CTCs in
blood samples obtained from patients with breast cancer. Our acoustic-based
separation method thus offers the potential to serve as an invaluable
supplemental tool in cancer research, diagnostics, drug efficacy assessment,
and therapeutics owing to its excellent biocompatibility, simple design, and
label-free automated operation while offering the capability to isolate rare
CTCs in a viable state11.
In 2015 , Long
Pang, Shaofei Shen, Chao Ma et al design a micro floudic
devices for cancer cell separation based on size 12.
In 2015 ,Liu, Yeonju Lee , Joon hee Jang et al developed one microfloudics devices used
for Microfluidic cytometric analysis of cancer cell transportability and
In 2015, Ryan M.
Williams and Letha J describes the
Selection of MREs using differential cell SELEX represents a powerful method of
differentiating between cell types. This process requires no prior knowledge of
cell surface molecule expression and takes advantage of differential expression
profiles. Peptide and antibody fragment libraries, the host on which the
library is displayed, and the selection method each have advantages and
situations in which they are useful. As more MREs are being developed, it is
certain that their uses and clinical investigation will continue to expand14.
In 2015, A. C. S. Talari, C. A. Evans et al showed that
Raman spectra of the cell lines have revealed that basic differences in the
concentration of biochemical compounds such as lipids, nucleic acids and
protein Raman peaks were found to differ in intensity, and principal component
analysis (PCA) was able to identify variations that lead to accurate and
reliable separation of the three cell lines. Linear discriminant analysis (LDA)
model of three cell lines was predicted with 100% sensitivity and 91%
specificity. We have shown that a combination of Raman spectroscopy and
chemometrics are capable of differentiation between breast cancer cell lines.
These variations may be useful in identifying new spectral markers to
differentiate different subtypes of breast cancer although this needs
confirmation in a larger panel of cell lines as well as clinical material 15.
In 2014 Nedhi at el explores the current
application of MEMS in cell reorganization and their DNA analysis 16.
In 2014 , Su S, Liu Q, Chen J et al showed
that mesenchymal-like breast cancer cells activate macrophages to a
TAM-like phenotype by GM-CSF. Reciprocally, CCL18 from TAMs induces cancer cell
EMT, forming a positive feedback loop, in coculture systems and humanized mice.
Inhibition of GM-CSF or CCL18 breaks this loop and reduces cancer metastasis 17.
In 2014 Ensieh
Farahani Hirak K. Patra Jaganmohan R.
Jangamreddy et al draw
comparison between reprogramming and carcinogenesis, as well as between stem
cells (SCs) and cancer stem cells (CSCs), focusing on changing garniture of
adhesion molecules. They also elaborate
on the role of adhesion molecules in the regulation of (cancer) SCs division
(symmetric or asymmetric), and in evolving interactions between CSCs and extracellular
In 2014, Adeeti V. Ullal, Vanessa Peterson et
al. describes that Cancer cell profiling by bar coding allows
multiplexed protein analysis in fine needle aspirates 19.
In 2014 Shailender Singh Kanwar,
Christopher James Dunlay et al. design and implementation of on chip micro
floudic devices for isolation, quantification and characterization of
circulating exosomes for cancer cell 20.